HuntingtIn
Western blot analysis of extracts of various cell lines, using Huntingtin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | MAB-94596 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | D7F7 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB, IHC, IF Western Blot: 1:500-1:2000 Immunohistochemistry (Paraffin): 1:50-1:200 Immunofluorescence: 1:50-1:200 |
| Molecular Weight: | 350 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1220 of human huntingtin protein. |
| Form: | Liquid |
Applications
WB, IHC, IF Western Blot: 1:500-1:2000 Immunohistochemistry (Paraffin): 1:50-1:200 Immunofluorescence: 1:50-1:200