Immunological Sciences

IBA1 Rabbit Monoclonal Antibody

Western blot analysis of different tissue lysates using rabbit mAb to IBA1, IBA1, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain, [4] mouse spleen, and [5] rat spleen. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates. The other bands seen in the CNS lysates are of unknown origin but do not appear to compromise the migroglial specific staining seen with this antibody.

Western blot analysis of different tissue lysates using rabbit mAb to IBA1, IBA1, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain, [4] mouse spleen, and [5] rat spleen. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates. The other bands seen in the CNS lysates are of unknown origin but do not appear to compromise the migroglial specific staining seen with this antibody.

Catalog Number:MAB-94785
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:EPR16589
Immunogen:Peptide identical to the C-terminal of human IBA1 coupled to KLH
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1,000-2,000. Immunofluorescence:1:1,000-2,000 Immunocytochemistry: 1:1,000-2,000 Immunohistochemistry(paraffin-tissues): 1:1000 Immunohistochemistry (frozen-tissues): 1:1000 “Free floating”
Molecular Weight:17kDa
Purification:Aff.Pur.
Form:Liquid

Applications

Western Blot: 1:1,000-2,000. Immunofluorescence:1:1,000-2,000 Immunocytochemistry: 1:1,000-2,000 Immunohistochemistry(paraffin-tissues): 1:1000 Immunohistochemistry (frozen-tissues): 1:1000 “Free floating”

Immunogen

Peptide identical to the C-terminal of human IBA1 coupled to KLH