IL10 Rabbit Polyclonal Antibody
Western blot analysis of various lysates, using IL10 Rabbit pAb at 1:800 dilution. Raw264. 7 cells were treated with LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.
| Catalog Number: | ABE3868 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:500 - 1:1000 IHC-P 1:50 - 1:100 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 18kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | CSIF; TGIF; GVHDS; IL-10; IL10A; IL10 |
Applications
WB 1:500 - 1:1000 IHC-P 1:50 - 1:100 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.