IL6 RAbbit Polyclonal Antibody

Western blot analysis of various lysates, using IL6 Rabbit pAb  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 90s.

Western blot analysis of various lysates, using IL6 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-84785
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 30-212 of human IL6
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:24 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2; IL6

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 30-212 of human IL6