Insulin Rabbit Polyclonal Antibody

Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution.

Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.

Lysates/proteInsulin: 25ug per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL West Pico Plus.

Exposure time: 30s.

Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteInsulin: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:AB-84377
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS.
Reactivity: Human, Mouse, Rat
Applications:<strong>Western Blot:                         </strong>1:500 - 1:2000 <strong>Immunohistochemistry:         </strong>1:50 - 1:200 <strong>Immunofluorescence:             </strong>1:50 - 1:200
Molecular Weight: 12kDa
Purification:Affinity purification
Form:Liquid
Synonyms:IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin

Applications

<strong>Western Blot:                         </strong>1:500 - 1:2000 <strong>Immunohistochemistry:         </strong>1:50 - 1:200 <strong>Immunofluorescence:             </strong>1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS.