Insulin Rabbit Polyclonal Antibody

Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteInsulin: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.
| Catalog Number: | AB-84377 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | <strong>Western Blot: </strong>1:500 - 1:2000 <strong>Immunohistochemistry: </strong>1:50 - 1:200 <strong>Immunofluorescence: </strong>1:50 - 1:200 |
| Molecular Weight: | 12kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin |
Applications
<strong>Western Blot: </strong>1:500 - 1:2000 <strong>Immunohistochemistry: </strong>1:50 - 1:200 <strong>Immunofluorescence: </strong>1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS.
