Insulin receptor (INSR)

Western blot analysis of extracts of HeLa cells, using INSR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Femto Plus Kit .
Exposure time: 120s.

Western blot analysis of extracts of HeLa cells, using INSR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Femto Plus Kit . Exposure time: 120s.

Catalog Number:AB-83500
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50-1:200.
Molecular Weight:Calculated MW: 155kDa/156kDa Observed MW: 156kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:INSR; CD220; HHF5; insulin receptor

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50-1:200.