Interleukin-1 Beta Rabbit Monoclonal Antibody
Western blot analysis of extracts of THP-1 cells, using IL1 beta antibody at 1:1000 dilution.THP-1 cells were treated by LPS (1 μg/mL) at 37℃ for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.
| Catalog Number: | MAB-94634 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | MI1 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 – 1:200 Immunohistochemistry (paraffin-embedded tissues) 1:100-300 |
| Molecular Weight: | 17kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B |
Applications
Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 – 1:200 Immunohistochemistry (paraffin-embedded tissues) 1:100-300
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230