Interleukin-10

Western blot analysis of extracts of
Jurkat cells, using IL10 antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.  Detection: ECL West Pico Plus. 
Exposure time: 10s.

Western blot analysis of extracts of Jurkat cells, using IL10 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-83616
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10.
Reactivity:Hu, Ms, Rt,
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100
Molecular Weight:22kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CSIF;GVHDS;IL-10;IL10A;TGIF;IL10

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10.