CD25 Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CD25 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.
| Catalog Number: | AB-81365 |
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD25. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Flow Cytometry: C 1:50 - 1:200 |
| Molecular Weight: | 60KDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | p55; CD25; IL2R; IMD41; TCGFR; IDDM10 |
Applications
Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Flow Cytometry: C 1:50 - 1:200
Immunogen
Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD25.