Interleukin-6

Western blot analysis of extracts of various cell lines, using IL6 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.Exposure time: 90.

Western blot analysis of extracts of various cell lines, using IL6 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.Exposure time: 90.

Catalog Number:AB-83767
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6
Reactivity:Hu,Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Mmunohistochemistry: 1:50 - 1:200
Molecular Weight:23kDa
Purification:Aff.Pur.
Form:Liquid
Synonyms:IL6;BSF-2;BSF2;CDF;HGF;HSF;IFN-beta-2;IFNB2;IL-6

Applications

Western Blot: 1:500 - 1:2000 Mmunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6