Interleukin-6
Western blot analysis of extracts of various cell lines, using IL6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.
| Catalog Number: | MAB-94635 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | I621 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human IL6. AA range:131-180 |
| Reactivity: | Hu,Ms, Rt |
| Applications: | Western Blot: 1/500 - 1/2000. Immunohistochemistry (paraffin-ebedded tissues): 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000 |
| Molecular Weight: | 23kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
Applications
Western Blot: 1/500 - 1/2000. Immunohistochemistry (paraffin-ebedded tissues): 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human IL6. AA range:131-180