Ki-67

Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.
| Catalog Number: | AB-84220 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant protein of human Ki67 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 |
| Molecular Weight: | 358kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of prolif eration Ki-67;Ki67 |
Applications
Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Immunogen
Recombinant protein of human Ki67
