Ki-67
![Western blot analysis of equal amounts of cell lysates probed with
chicken pAb to Ki67, Ki67, dilution 1:5,000, in green: [1]
protein standard (red), [2] rapidly dividing HeLa cell cultures, [3]
rapidly dividing HEK293 cell cultures. Strong double bands above
250kDa correspond to the two major Ki67 isoforms of molecular
weight 345kDa and 395kDa. Since Ki67 is a rather unstable snd
sjhort half life protein multiple proteolytic fragments of the two
isoforms are also detected on the blot.](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83925-1.jpg)
Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, Ki67, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.
| Catalog Number: | AB-83925 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ch |
| Isotype: | IgY |
| Clone: | POLY |
| Immunogen: | Mixture of recombinant human Ki67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490 |
| Reactivity: | Hu Rt Ms,Mk |
| Applications: | Western Blot: 1:2,500 Immunofluorescence: 1:1,000-2,500 Immunocytochemistry: 1:1,000-2,500 |
| Molecular Weight: | 45kDa and 395kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
Applications
Western Blot: 1:2,500 Immunofluorescence: 1:1,000-2,500 Immunocytochemistry: 1:1,000-2,500
Immunogen
Mixture of recombinant human Ki67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490