Lamin A/C

Western blot analysis of extracts of various cell lines, using Lamin A/C Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using Lamin A/C Rabbit pAb at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Catalog Number:AB-90019
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 ChIP: 1:20 - 1:100
Molecular Weight:63KDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:LMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; ;LMN1;LMNC;LMNL1;MADA;PRO1;lamin;Lamin A/C

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 ChIP: 1:20 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C.