LC3B Polyclonal Antibody
Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody at 1:1000 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.
| Catalog Number: | AB-84447 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohsitochemistry: 1:50 - 1:200 Immunofluorescence / Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 |
| Molecular Weight: | 14KDa/16KDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B |
Applications
Western Blot: 1:500 - 1:2000 Immunohsitochemistry: 1:50 - 1:200 Immunofluorescence / Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B.