LOX Rabbit mAb

Western blot analysis of various lysates using LOX Rabbit mAb  at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Western blot analysis of various lysates using LOX Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Catalog Number:AB11504
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC0624
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Reactivity:Human,Mouse,Rat
Applications:WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:56kDa
Purification:Affinity purification
Form:liquid
Synonyms:AAT10; LOX

Applications

WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive.