MAP-2ab
Western blot - Anti-MAP2 antibody All lanes : Anti-MAP2 antibody at 1/50000 dilution Lane 1 : Adult rat brain lysate Lane 2 : Embryonic E20 rat brain lysate Lane 3 : Adult mouse brain lysate
| Catalog Number: | AB-10417 |
| Conjugate: | Unconjugated |
| Size: | 50 ul |
| Concentration: | 1mg/ml |
| Host: | Ch |
| Isotype: | IgY |
| Clone: | POLY |
| Immunogen: | Full length native protein (purified) corresponding to Cow MAP2. Positive control: ICC: cultured rat cortical neurons.Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. |
| Reactivity: | Hu, Rt, Ms, Bv, Mamm |
| Applications: | Western Blot: 1:10000-1:100.000 This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. Immunocytochemistry: 1:5000-10.000 Immunofluorescence: 1:5000-10.000 Immunohistochemistry (frozen tissues)1:5000-10.000 Immunohistochemistry (paraffin-embedded tissues:To be optimized by end user. ELISA: 1:5000 |
| Molecular Weight: | ~280kDa |
| Purification: | Serum |
| Form: | Liquid |
Applications
Western Blot: 1:10000-1:100.000 This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. Immunocytochemistry: 1:5000-10.000 Immunofluorescence: 1:5000-10.000 Immunohistochemistry (frozen tissues)1:5000-10.000 Immunohistochemistry (paraffin-embedded tissues:To be optimized by end user. ELISA: 1:5000
Immunogen
Full length native protein (purified) corresponding to Cow MAP2. Positive control: ICC: cultured rat cortical neurons.Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.