MAP-2a,b,c,d
![Western blot analysis of tissue and cell lysates using mouse mAb to MAP2C/D, MAP2 A/B/C/D , dilution 1:5,000 in green, and chicken pAb to vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293 and [6] NIH-3T3 cell lysates. A band at about 280kDa is full length intact MAP2A/2B isotypes while bands at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. The Vim antibody binds to a single band at ~50kDa present in all preparations.](https://immunologicalsciences.com/wp-content/uploads/2022/01/MAB-83910-MAP2A-B-C-D-1.jpg)
Western blot analysis of tissue and cell lysates using mouse mAb to MAP2C/D, MAP2 A/B/C/D , dilution 1:5,000 in green, and chicken pAb to vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293 and [6] NIH-3T3 cell lysates. A band at about 280kDa is full length intact MAP2A/2B isotypes while bands at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. The Vim antibody binds to a single band at ~50kDa present in all preparations.
| Catalog Number: | MAB-83910 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Clone: | 2C4 |
| Immunogen: | Full length recombinant human MAP2D protein Prot-r-MAP2D expressed in and purified from E. coli. |
| Reactivity: | Hu Rt Ms |
| Applications: | Western Blot: 1:5,000-1:10,000 Immunohistochemistry: 1:5,000-10,000 Immunofluorescence: 1:5,000-10,000 Immunocytochemistry: 1:5,000-10,000 |
| Molecular Weight: | MAP2A/B ~280 kDa, MAP2C/D ~70kDa by SDSPAGE |
| Purification: | Purified |
| Form: | Liquid |
Applications
Western Blot: 1:5,000-1:10,000 Immunohistochemistry: 1:5,000-10,000 Immunofluorescence: 1:5,000-10,000 Immunocytochemistry: 1:5,000-10,000
Immunogen
Full length recombinant human MAP2D protein Prot-r-MAP2D expressed in and purified from E. coli.