MAP1LC3A / MAP1LC3B Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 3min.
| Catalog Number: | AB-83557 |
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-60 of human LC3A |
| Reactivity: | Human,Mouse, Rat |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry(paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 14-16kDa |
| Purification: | Purified |
| Form: | Liquid |
| Synonyms: | MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;MAP1LC3A;ATG8E;LC3;LC3A;MAP1ALC3;MAP1BLC3;LC3A/LC3B |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry(paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: Immunocytochemistry: 1:50 - 1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-60 of human LC3A