MAP1LC3b
Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 30s..
| Catalog Number: | AB-83629 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50-1:200 Immunofluorescence: 1:20-1:200 |
| Molecular Weight: | 14kDa |
| Purification: | Purified |
| Form: | Liquid |
| Synonyms: | ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a |
Applications
Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50-1:200 Immunofluorescence: 1:20-1:200