MAP1LC3b
Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody at 1:1000 dilution. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane Blocking buffer: 3% nonfat dry milk inTBST Detection: ECL West Pico Plus Exposure time: 3min.
| Catalog Number: | AB-84132 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100 |
| Molecular Weight: | 16kDa |
| Purification: | Purified |
| Form: | Liquid |
| Synonyms: | MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta |
Applications
Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B