mTOR
Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Femto Plus Exposure time: 15s
| Catalog Number: | AB-83580 |
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR |
| Reactivity: | Hu,Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Immunoprecipitation: 1:50 |
| Molecular Weight: | Calculated MW: 288kDa Observed MW: 290kDa |
| Purification: | Aff, Pur. |
| Form: | Liquid |
| Synonyms: | MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin |
Applications
Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Immunoprecipitation: 1:50
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR