Myogenin Rabbit Polyclonal Antibody

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit 
Exposure time: 30s.

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 30s.

Catalog Number:AB6664
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:Recombinant protein.This information is considered to be commercially sensitive.
Reactivity:Human,Mouse,Rat
Applications:WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:34kDa
Purification:Affinity purification
Form:liquid
Synonyms:MYF4; myf-4; bHLHc3; Myogenin

Applications

WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Recombinant protein.This information is considered to be commercially sensitive.