Nestin Rabbit Polyclonal Antibody
Western blot analysis of lysates from Neuro-2a cells, using Nestin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.
| Catalog Number: | AB-84800 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant Protein corresponding to a sequence within amino acids 1392-1621 of human NES. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 150kDa/177kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | Nbla00170; Nestin |
Applications
Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Immunogen
Recombinant Protein corresponding to a sequence within amino acids 1392-1621 of human NES.