Immunological Sciences

Neurofilament Heavy (NF-H)

Western blot detection of the heavily phosphorylated axonal form of NFH protein (pNF-H) in neural tissue lysates (20μg/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (AH1) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain. Adult rat spinal cord Adult mouse whole brain. Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.

Western blot detection of the heavily phosphorylated axonal form of NFH protein (pNF-H) in neural tissue lysates (20μg/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (AH1) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain. Adult rat spinal cord Adult mouse whole brain. Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.

Catalog Number:MAB-94404
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG1
Clone:AH1
Immunogen:Native NF-H purified from bovine spinal cord
Reactivity:Hu Rt Ms,Cw, Pg, horse
Applications:Western Blot: 1:10,000 Immunocytochemistry: 1:1,000 Immunofluorescence: 1:1,000 Immunohistochemistry: 1:1,000
Molecular Weight:200-220 kDa
Purification:Purified
Form:Liquid

Applications

Western Blot: 1:10,000 Immunocytochemistry: 1:1,000 Immunofluorescence: 1:1,000 Immunohistochemistry: 1:1,000

Immunogen

Native NF-H purified from bovine spinal cord