Neurofilament Heavy (NF-H) (Phosphorylated)

Western blot analysis of tissue lysates using mouse mAb to NF-H, SMI-31, dilution 1:10,000 in green: protein standard (red), rat brainrat spinal cord, mouse brain, mouse spinal cord, pig spinal cordcow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with SMI-31 antibody.