Immunological Sciences

Neurofilament Medium (NF-M)

Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NF-M, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NF-M, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

Catalog Number:AB-10686
Conjugate:Unconjugated
Size:50 ul
Concentration:1mg/ml
Host:Ch
Isotype:IgG
Clone:POLY
Immunogen:Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.
Reactivity:Hu, Rt, Ms, Ch
Applications:Western Blot: 1:2,000-5,000 Immunofluorescence: 1:500-1,000 Immunocytochemistry: 1:500-1,000 Immunohistochemistry: 1:500-1,000
Molecular Weight:145-160kDa by SDSPAGE
Purification:Serum
Form:Liquid

Applications

Western Blot: 1:2,000-5,000 Immunofluorescence: 1:500-1,000 Immunocytochemistry: 1:500-1,000 Immunohistochemistry: 1:500-1,000

Immunogen

Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.