NFKB1 Rabbit Monoclonal antibody
Western blot analysis of various lysates using NFKB1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 10 s.
| Catalog Number: | AB27463 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse |
| Applications: | WB 1:1000 - 1:2000 IHC-P 1:200 - 1:400 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL ChIP 5μg antibody for 5μg-20μg of Chromatin. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 50 kDa(Active form)/120 kDa(Precursor) |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta |
Applications
WB 1:1000 - 1:2000 IHC-P 1:200 - 1:400 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL ChIP 5μg antibody for 5μg-20μg of Chromatin. Please optimize the concentration based on your specific assay requirements.
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.