NG2/CSPG4 Rabbit Monoclonal Antibody

Western blot analysis of lysates from Mouse ovary using NG2/CSPG4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

Western blot analysis of lysates from Mouse ovary using NG2/CSPG4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:MAB-94805
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC64580
Immunogen:Recombinant protein.This information is considered to be commercially sensitive.
Reactivity:Human,Mouse
Applications:WB 1:1000 - 1:2000 IF-P 1:200 - 1:1000 IHC-P 1:500 - 1:2000 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:450kDa
Purification:Affinity purification
Form:liquid
Synonyms:NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; NG2/CSPG4

Applications

WB 1:1000 - 1:2000 IF-P 1:200 - 1:1000 IHC-P 1:500 - 1:2000 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Recombinant protein.This information is considered to be commercially sensitive.