NLRP3
Western blot analysis of RAW264.7, using NLRP3 antibody at 1:770 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.
| Catalog Number: | AB-84728 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of mouse NLRP3. |
| Reactivity: | Human, Mouse |
| Applications: | Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 110KDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | AII; AVP; FCU; MWS; FCAS; KEFH; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; DFNA34; PYPAF1; AGTAVPRL |
Applications
Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of mouse NLRP3.