NLRP3 Rabbit Monoclonal Antibody
Western blot analysis of lysates from RAW 264.7 cells, using NLRP3 Rabbit mAb at 1:1000 dilution. Raw264. 7 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) ) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | MAB24294 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC62768 |
| Immunogen: | Recombinant protein.This information is considered to be commercially sensitive. |
| Reactivity: | Mouse |
| Applications: | WB 1:1000 - 1:6000 IF/ICC 1:100 - 1:500 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 118kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL; NLRP3 |
Applications
WB 1:1000 - 1:6000 IF/ICC 1:100 - 1:500 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant protein.This information is considered to be commercially sensitive.