NOTCH 1 Rabbit Polyclonal Antibody

Western blot analysis of various lysates using Notch1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.
| Catalog Number: | AB-90186 |
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Immunogen A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1. |
| Reactivity: | Human, Mouse |
| Applications: | Western Blot: 1:500 - 1:2000 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Molecular Weight: | 125kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | hN1; AOS5; TAN1; AOVD1; Notch1 |
Applications
Western Blot: 1:500 - 1:2000 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Immunogen
Immunogen A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1.
