NOTCH 1 Rabbit Polyclonal Antibody

Western blot analysis of various lysates using Notch1 Rabbit pAb  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   
Exposure time: 3min.

Western blot analysis of various lysates using Notch1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:AB-90186
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Immunogen A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1.
Reactivity:Human, Mouse
Applications:Western Blot: 1:500 - 1:2000 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Molecular Weight:125kDa
Purification:Affinity purification
Form:Liquid
Synonyms:hN1; AOS5; TAN1; AOVD1; Notch1

Applications

Western Blot: 1:500 - 1:2000 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Immunogen

Immunogen A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1.