NOX2
Western blot analysis of extracts of various cell lines, using Nox2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit Exposure time: 60s.
| Catalog Number: | AB-83889 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | poly |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 283-570 of human Nox2 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1 :500 – 1:1000 Immunohistochemistry (paraffine Embedded tissues) : 1:50-1:100 Immunofluorescence: 1:50-1:100 |
| Molecular Weight: | 65kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | CYBB; AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX; cytochrome b-245 beta chain |
Applications
Western Blot: 1 :500 – 1:1000 Immunohistochemistry (paraffine Embedded tissues) : 1:50-1:100 Immunofluorescence: 1:50-1:100
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 283-570 of human Nox2