NRF2 Rabbit Polyclonal Antibody

Western blot analysis of lysates from
1)Hela cell ,  
2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUC
AAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. 
(Red) GAPDH Monoclonal Antibody (5B7)
antibody was diluted at 1:5000 as loading control, 4° over night, AF680 secondary antibody was diluted at 1:10000, 37° 1hour.

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUC AAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4° over night, AF680 secondary antibody was diluted at 1:10000, 37° 1hour.

Catalog Number:AB-84017
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605.
Reactivity:Hu, Ms,Rt
Applications:Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300Immunofluorescence: 1/200 - 1/1000ELISA: 1/40000
Molecular Weight:75-100kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form:Liquid
Synonyms:NFE2L2; NRF2; Nuclear factor erythroid 2-relatedfactor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2

Applications

Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300Immunofluorescence: 1/200 - 1/1000ELISA: 1/40000

Immunogen

The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605.