NRF2 Rabbit Polyclonal Antibody

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUC AAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4° over night, AF680 secondary antibody was diluted at 1:10000, 37° 1hour.
| Catalog Number: | AB-84017 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605. |
| Reactivity: | Hu, Ms,Rt |
| Applications: | Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300Immunofluorescence: 1/200 - 1/1000ELISA: 1/40000 |
| Molecular Weight: | 75-100kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form: | Liquid |
| Synonyms: | NFE2L2; NRF2; Nuclear factor erythroid 2-relatedfactor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2 |
Applications
Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300Immunofluorescence: 1/200 - 1/1000ELISA: 1/40000
Immunogen
The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605.
