p38 MAP Kinase
Westen blot analysis of extracts of various cell lines, using p38 MAPK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.
| Catalog Number: | AB-83572 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blot: 1:1000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:100 Immunoprecipitation: 1:50-1:100 |
| Molecular Weight: | 41kDa |
| Purification: | Polyclonal antibodies are producedby immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography. |
| Form: | Liquid |
Applications
Western blot: 1:1000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:100 Immunoprecipitation: 1:50-1:100