P53
Western blot analysis of extracts of various cell lines, using p53 antibody at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 15s.
| Catalog Number: | AB-83528 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p53. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:3000 - 1:6000 Immunohistochemistry: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:50 ChIP: 1:20 - 1:50 |
| Molecular Weight: | 53kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
Applications
Western Blot: 1:3000 - 1:6000 Immunohistochemistry: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:50 ChIP: 1:20 - 1:50
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p53.