p53
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| Catalog Number: | MAB-10166 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG2a |
| Clone: | Bp53.12 |
| Immunogen: | Bacterially expressed full-length wild-type p53 |
| Reactivity: | Hu |
| Applications: | Flow Cytometry Immunoprecipitation Western Blotting Recommended dilution: 1-2 µg/ml, overnight in 4oC Positive control: RAMOS human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel). Immunohistochemistry (paraffin sections) Immunocytochemistry: 2-10ug/ml ELISA |
| Purification: | Purified from ascites by precipitation methods |
| Form: | Liquid |
Applications
Flow Cytometry Immunoprecipitation Western Blotting Recommended dilution: 1-2 µg/ml, overnight in 4oC Positive control: RAMOS human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel). Immunohistochemistry (paraffin sections) Immunocytochemistry: 2-10ug/ml ELISA
Immunogen
Bacterially expressed full-length wild-type p53