Parvalbumin Mouse Monoclonal Antibody

Western blot analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse mAb to parvalbumin, MAB-94390 dilution 1:1,000 in green: [1] protein standard (red), [2] mouse
muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged
recombinant parvalbumin protein lane as expected since the His-tag
and other vector derived sequence adds about 6kDa to the
molecule. Note that the MAB-94390antibody is not cross-reactive with
either calbindin or calretinin despite their related amino acid
sequences.

Western blot analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse mAb to parvalbumin, MAB-94390 dilution 1:1,000 in green: [1] protein standard (red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the MAB-94390antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.

Catalog Number:MAB-94390
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1
Clone:3C9
Immunogen:Full-length recombinant human protein expressed in and purified from E. coli.
Reactivity:Hu Rt Ms
Applications:Western Blot: 1:1,000-1:5,000 Immunocytochemistry: 1:1,000-1:5,000 Immunofluorescence: 1:1,000-1:5,000 Immunohistochemistry: 1:1,000-1:5,000
Molecular Weight:12kDa
Purification:Purified
Form:Liquid

Applications

Western Blot: 1:1,000-1:5,000 Immunocytochemistry: 1:1,000-1:5,000 Immunofluorescence: 1:1,000-1:5,000 Immunohistochemistry: 1:1,000-1:5,000

Immunogen

Full-length recombinant human protein expressed in and purified from E. coli.