Paxillin Mouse Monoclonal Antibody

Western blot analysis of Paxillin using anti-Paxillin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates Lane 2: human Hela whole cell lysates Lane 3: human MCF-7 whole cell lysates Lane 4: human CACO-2 whole cell lysates Lane 5: rat liver tissue lysates Lane 6: rat RH35 whole cell lysates Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes.Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Paxillin antigen affinity purified monoclonal antibody at 1 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Paxillin at approximately 65 kDa. The expected band size for Paxillin is at 65 kDa.