PCNA Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.
| Catalog Number: | AB-81598 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110 |
| Reactivity: | Human;Mouse;Rat;Monkey;Rabbit |
| Applications: | Western Blot: 1:500-2000 Immunohistochemistry(paraffin-embedded tissues): 1:100-500 Immunofluorescence: 1:100-500 Immunocytochemisty: 100-500 ELISA 1:5000-20000 |
| Molecular Weight: | 36kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin |
Applications
Western Blot: 1:500-2000 Immunohistochemistry(paraffin-embedded tissues): 1:100-500 Immunofluorescence: 1:100-500 Immunocytochemisty: 100-500 ELISA 1:5000-20000
Immunogen
The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110