PD-L1 (CD274) Rabbit Polyclonal Antibody

Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody  at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-84131
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 131-239 of human PD-L1/CD274
Reactivity:Human, Mouse
Applications:Western Blot:                     1:500 - 1:1000Immunohistochemistry:  1:50 - 1:200
Molecular Weight:40KDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CD274; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; CD274 molecule

Applications

Western Blot:                     1:500 - 1:1000Immunohistochemistry:  1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 131-239 of human PD-L1/CD274