PD-1 Rabbit Polyclonal Antibody
Western blot analysis of various lysates using PD-1/CD279 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.
| Catalog Number: | AB-84286 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-259 of human PD-1/CD279. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 ELISA Recommended startingconcentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 50kDa/35kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD-1/CD279 |
Applications
Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 ELISA Recommended startingconcentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 205-259 of human PD-1/CD279.