PDK1

Western blot analysis of extracts of various cell lines, using PDK1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico. .
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using PDK1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico. . Exposure time: 90s.

Catalog Number:AB-90230
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1.
Reactivity:HU, Ms,
Applications:Western Blot: 1:500 -1:1000 Immunohistochemistry: 1:50 -1:100 Immunofluorescence: 1:50 - 1:100
Molecular Weight:68kDa
Purification:Purified
Form:Liquid
Synonyms:PDPK1; PDK1; PDPK2; PDPK2P; PRO0461; 3-phospho inositide-dependent protein kinase 1

Applications

Western Blot: 1:500 -1:1000 Immunohistochemistry: 1:50 -1:100 Immunofluorescence: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1.