Phospho-AKT1 (S473)
A) Western blot analysis of Akt activation using AB10521 Phospho Ser 473 AKT in striatal neurons stimulated with DHPG (mGluR5 agonist) for 0 (lane 1), 2 (lane 2), 5 (lane 3) or 10 (lane 4) min. B) Western blot analysis of total Akt (detected with anti-Akt1 antibody, AB-10521 in striatal neurons stimulated with DHPG (a mGluR5 agonist) for 0 (lane 1), 2 (lane 2), 5 (lane 3) or 10 (lane 4) min. Wells were equally loaded with 100 μg of whole cell lysate proteins.
| Catalog Number: | AB-10521 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Peptide surrounding pSer-473 at the C-terminal sequence of human AKT protein |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB: 1:1,000 ICC/IF: 1:500 IHC: 1:500 |
| Molecular Weight: | 60 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt1 protein. |
| Form: | liquid |
| Synonyms: | AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1 |
Applications
WB: 1:1,000 ICC/IF: 1:500 IHC: 1:500
Immunogen
Peptide surrounding pSer-473 at the C-terminal sequence of human AKT protein