Phospho-AKT1 (S473) Rabbit Monoclonal Antibody
Western blot analysis of extracts from Akt1 (-/-) mouse embryonic fibroblast (MEF) or Akt2 (-/-) MEF, untreated or stimulated with hPDGF (100 ng/ml, 15 min), using Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific) (upper), Phospho-Akt (Ser473) (D9E) Rabbit mAb (middle), or Akt (pan) (C67E7) Rabbit (lower).
| Catalog Number: | MAB-94125 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | D7F10 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473. AA range:431-480 |
| Reactivity: | Human;Mouse;Rat;Chicken |
| Applications: | Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 |
| Molecular Weight: | 60 kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | liquid |
| Synonyms: | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; |
Applications
Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473. AA range:431-480