Immunological Sciences

Phospho-EGFR (T1068)

Western blot analysis of extracts of various cell lines extracts of A-431 and HeLa cells, using Phospho-EGFR-Y1068 antibody at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25ug per lane Blocking buffer: 3% BSA. Detection: ECLPico Kit Exposure time: 1min.

Western blot analysis of extracts of various cell lines extracts of A-431 and HeLa cells, using Phospho-EGFR-Y1068 antibody at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25ug per lane Blocking buffer: 3% BSA. Detection: ECLPico Kit Exposure time: 1min.

Catalog Number:ABP-0301
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Reactivity:Hu, Ms
Applications:Western blotting 1:1000
Molecular Weight:175 kDa
Purification:Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography.
Form:liquid

Applications

Western blotting 1:1000