Immunological Sciences

Phospho-EGFR (T1068)

Phospho-EGFR (Tyr1068) Western blot analysis of extracts of various cell lines extracts of A431 and HeLa cells, using Phospho-EGFR-Tyr-1068 antibody at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000dilution. Lysates/proteins: 25ug per lane Blocking buffer: 3% BSA. Detection: ECLPico Kit Exposure time: 1min.

Phospho-EGFR (Tyr1068) Western blot analysis of extracts of various cell lines extracts of A431 and HeLa cells, using Phospho-EGFR-Tyr-1068 antibody at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000dilution. Lysates/proteins: 25ug per lane Blocking buffer: 3% BSA. Detection: ECLPico Kit Exposure time: 1min.

Catalog Number:MAB-94208
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Reactivity:Hu, Ms
Applications:WB: 1:1000
Molecular Weight:175 kDa
Purification:Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor.
Form:liquid

Applications

WB: 1:1000