Phospho-EGFR (Y992)

Fig. 1. Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reducing conditions).
| Catalog Number: | MAB-90139 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Clone: | EM-12 |
| Immunogen: | Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR. |
| Reactivity: | Hu |
| Applications: | Flow Cytometry Immunoprecipitation Western Blotting Recommended dilution: 1 µg/ml Positive control: EGF-stimulated A431 Negative control: Non-stimulated A431 |
| Purification: | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Form: | liquid |
Applications
Flow Cytometry Immunoprecipitation Western Blotting Recommended dilution: 1 µg/ml Positive control: EGF-stimulated A431 Negative control: Non-stimulated A431
Immunogen
Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR.
