Phospho-ERK1/ERK2 (p44/p42 MAPK) (T202/Y204)
Detection of ERK1 and ERK2 phosphorylated at T202/Y204 and T185/Y187, respectively. Human HeLa cells were incubated with 200 nM PMA for the indicated times. Total cell lysates in gel sample buffer were resolved by SDS-PAGE, transferred to Immobilon membrane and Immunoblotted with 0.5µg/Ml antiphosphERK1/2. phosphoERK1/2
| Catalog Number: | AB-10762-A |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting: 1:2000 Immunohistochemistry: 1:100-500 Immunofluorescence: 1:100-500 Immunoprecipitation: to be determined Immunocytochemistry: 1:100 Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator. Recognizes ERK1 and ERK2 dually phosphorylated at T202/Y204 and T185/Y187, respectively. |
| Form: | liquid |
Applications
Western blotting: 1:2000 Immunohistochemistry: 1:100-500 Immunofluorescence: 1:100-500 Immunoprecipitation: to be determined Immunocytochemistry: 1:100 Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator. Recognizes ERK1 and ERK2 dually phosphorylated at T202/Y204 and T185/Y187, respectively.
Immunogen
Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2