Phospho-ERK1-T202/Y204 + ERK2-T185/Y187
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 antibody at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.
| Catalog Number: | ABP-0472 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | A synthetic phosphorylated peptide around T185/Y187 of human ERK2. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100 |
| Molecular Weight: | 42KDa/44KDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | MAPK1/MAPK3 |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Immunogen
A synthetic phosphorylated peptide around T185/Y187 of human ERK2.